Fluorescence Studies on the Interaction between Pyridoxal Phosphate Enzymes

Fluorescence studies on the interaction between pyridoxal phosphate enzymes.

Mechanistic Studies on the Pyridoxal Phosphate Enzyme

The enzyme 1 -aminocyclopropane1 -carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to a-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 18251. Enzymatic incubations in D20 yield a-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of...

The reaction of amino-oxyacetate with pyridoxal phosphate-dependent enzymes.

The carbonyl reagent amino-oxyacetate is frequently used in metabolic studies to inhibit individual pyridoxal phosphate enzymes. The reaction of this compound with three such enzymes, aspartate transaminase, 4-aminobutyrate transaminase and dopa (3,4-dihydroxyphenylalanine) decarboxylase, was studied to determine the extent to which the inhibition is reversible and the rates at which it takes p...

Studies on the Interaction between Regulatory Enzymes and Effecters

In order to elucidate the chemical basis of interaction between regulatory enzymes and effecters, various structural analogues of adenosine 5’-monophosphate were investigated for the activating effect on the biodegradative threonine deaminase of Escherichia coli. The mode of activation by each compound was analyzed kinetically in terms of the maximal activation effect and the concentration whic...

PLPMDB: Pyridoxal-5'-phosphate dependent enzymes mutants database

SUMMARY The searchable mutant database PLPMDB has been developed to provide rapid and simple access to relevant mutant information on pyridoxal-5'-phosphate dependent enzymes. All data have been extracted from publications and publicly available databases, then organized in a relational database to enable searching via a web-based search form. The current version of PLPMDB contains 688 mutants ...